标题：Multilevel analysis of the impact of environmental factors and agricultural practices on the concentration in hay of microorganisms responsible for farmer's lung disease.
期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2009
卷号：16
期号：02
页码：219-225
出版社：Institute of Agricultural Medicine in Lublin
摘要：Farmer's lung disease (FLD) is common in eastern France. It is the main form of occupational hypersensitivity pneumonitis, caused by chronic inhalation of microor-ganisms (antigens) from mouldy hay, straw, or grain. The purpose of this study was to assess, with a panel of data collected between 1997–2003, environmental factors and agricultural practices that independently modify concentrations in hay of microorganisms potentially responsible for FLD. A total of 629 hay samples from 86 farms were included in statistical analyses using linear multilevel regression models allowing to consider the nested structure of the data: individual-level (batch of hay) and group-level (farm). The outcome variable of these models was the concentration in hay (logarithmic value of con-centration+1) of microorganisms incriminated in FLD (Absidia corymbifera, Eurotium spp., thermophilic actinomycetes). The simultaneous analysis of batch of hay- and farm-level factors showed that bad climatic conditions of harvest, high-density hay-packing modes, (especially round bales) and altitude (2ndplateau, ]700–900] m) were the main factors associated with high concentrations of these microorganisms in hay. This study allowed clarification of the factors that influence the microbial concentration of hay with etiological agents of FLD
