期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2009
卷号：16
期号：02
页码：313-319
出版社：Institute of Agricultural Medicine in Lublin
摘要：715 Ixodes ricinus ticks from 4 sites of Lublin macroregion (Piotrowice, Pole-sie National Park, D.browa and ..czyńsko-W.odawskie Lakeland) were examined for the presence of Borrelia burgdorferi sensu lato and Toxoplasma gondi, using polymer-ase chain reaction (Pcr) and nested-Pcr methods. The clonal type of samples tested positive for T. gondii was identified by amplification and fragmenting with restrictions enzymes (rFLP-restriction fragment length polymorphism). Positive results were con-firmed by sequencing. The overall percentage of tick infection with B. burgdorferi sensu lato (s.l.) was 12.7%, and with T. gondii – 12.6%. The coincidence of both pathogens was found in 2.4% of the examined specimens. The highest proportions of infections were detected in females, among all the developmental stages, both in the case of single pathogens (23.5% of ticks infected with B. burgdorferi s.l., and 19.7% – with T. gondii), and their coincidence (3.8% of all the tested females). rFLP Pcr test detected that type I and atypical (type A) T. gondii strains were most common in ticks. The presence of type II/III was confirmed exclusively in females
