期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2012
卷号：19
期号：2
页码：279-284
出版社：Institute of Agricultural Medicine in Lublin
摘要：The e ect of perpendicular and swirling aerozolization methods on the release of fungal fragments and spores from agar surface was studied. Three fungal species (Aspergillus versicolor, Cladosporium cladosporioides, Penicillium chrysogenum) were selected for the tests as they commonly occur indoors, create di erent hyphae structure when they grow on surfaces, and have di erent spore shapes, aerodynamic sizes, and formation mechanisms. As the tested surface, Petri dishes lled with malt extract agar, separately inoculated with fungal strains and cultivated to obtain an abundant and even growth were used. For the purpose of these experiments, a new aerosolization chamber was built in which HEPA- ltered air stream responsible for fungal propagule release was either perpendicularly directed towards the contaminated surface or set in swirling motion above it. The experiments were conducted at 2 air velocities, typical for outdoor environment (11.6 m/s) and ventilation ducts (29.1 m/s). Concentrations and size distributions of released fragments and spores were measured using an optical particle counter. The results showed that the propagule release depends on the direction (swirling motion was able to release up to 3.4×105fragments and 3×105spores from 1 cm2of contaminated surface, i.e. signi cantly more than the perpendicularly directed air stream), velocity (the higher the swirling air velocity applied, the higher the number of released propagules) of the air stream above the contaminated surface, and varied due to the taxonomical species origin (the higher number of particulates was released by Aspergillus colonies). Hence, the e cient control of both microbial fragments and spores, not only in the air, but also in their source should be an integral part of the quality control procedure
关键词：fungi; fragments; spores; agar surface; swirling release; air velocity
