期刊名称：Journal of Applied Sciences in Environmental Sanitation
印刷版ISSN：0126-2807
电子版ISSN：1978-6980
出版年度：2011
卷号：6
期号：2
页码：123-136
出版社：Sepuluh Nopember Institute of Technology (ITS)
摘要：Extracellular keratinase was produced during submerged aerobic cultivation in a medium containing chicken feather for enzyme synthesis. The enzyme was partially purified by acetone fractionation and DEAE Sephadex A 25 column chromatography. A purification fold about 20.7 with a yield of 37.9% as determined with keratin as substrate of the activity in crude extracts. Specific activity of this partially purified enzyme is 2312.7 U/mg. The kmand Vmaxvalues were 7.15 mM and 300U/ml respectively. The optimal pH and temperature for keratinolytic activity was approximately 7.0 and 70°C respectively. Essential amino acids like threonine, valine, methionine, isoleucine, leucine, lysine, histidine and tyrosine as well as ammonia were produced when feathers were used as substrates. Exposures of purified keratinase in absence of substrate at 80°C, for 60 minutes caused lose about 56% of its activity. This keratinase was inhibited in a variable rates by addition of EDTA, CuSO4, ZnCl2MnCl2 and HgCl2at a concentration of 15mM, where as iodoacetate and 2 mercaptoethanol slightly activation at the same concentration. Strain Aspergillus oryzae, therefore, shows great promise of finding potential applications in keratin hydrolysis and keratinase production
