This study is focused on finding a possible relation between deception and emotional intelligence among respondents in the Czech Republic. Emotional intelligence is related to self-deception and detection of lying, yet there is not enough information on its possible relation to lying behavior itself. A deception behavior survey and the TEIQue inventory were used to analyse deception characteristics and emotional intelligence among 112 respondents. ANOVA and regression analysis revealed significant differences in lying behavior, which might be influenced by the global trait of emotional intelligence or well-being. Results suggest that respondents with lower emotional intelligence use less lies and tend to lie to adults more often than to children. These results might be beneficial for interventions in pedagogical or forensic psychology or in family therapy and can improve our understanding of variables affecting lying behavior.