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  • 标题:Biotechnology and Conservation of Plant Biodiversity
  • 本地全文:下载
  • 作者:Carlos Alberto Cruz-Cruz ; María Teresa González-Arnao ; Florent Engelmann
  • 期刊名称:Resources
  • 电子版ISSN:2079-9276
  • 出版年度:2013
  • 卷号:2
  • 期号:2
  • 页码:73-95
  • DOI:10.3390/resources2020073
  • 语种:English
  • 出版社:MDPI Publishing
  • 摘要:Advances in plant biotechnology provide new options for collection, multiplication and short- to long-term conservation of plant biodiversity, using in vitro culture techniques. Significant progress has been made for conserving endangered, rare, crop ornamental, medicinal and forest species, especially for non-orthodox seed and vegetatively propagated plants of temperate and tropical origin. Cell and tissue culture techniques ensure the rapid multiplication and production of plant material under aseptic conditions. Medium-term conservation by means of in vitro slow growth storage allows extending subcultures from several months to several years, depending on the species. Cryopreservation (liquid nitrogen, −196 °C) is the only technique ensuring the safe and cost-effective long-term conservation of a wide range of plant species. Cryopreservation of shoot tips is also being applied to eradicate systemic plant pathogens, a process termed cryotherapy. Slow growth storage is routinely used in many laboratories for medium-conservation of numerous plant species. Today, the large-scale, routine application of cryopreservation is still restricted to a limited number of cases. However, the number of plant species for which cryopreservation techniques are established and validated on a large range of genetically diverse accessions is increasing steadily.
  • 关键词:biotechnology; conservation; plant biodiversity; in vitro collecting; slow growth storage; cryopreservation; endangered species
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