标题：Metabolomic Studies Using High Performance Liquid Chromatography and Tandem Mass Spectrometry to Discover Quality Markers for Oriental Beauty (Dongfang Meiren) Tea
摘要：In this study, a
high-performance liquid chromatograph and an electrospray ionization (ESI)
triple quadrupole mass spectrometry (TQ MS) detector were used to scan Oriental
Beauty tea of different grades and prices. Principle component analysis (PCA)
of the profiling data was performed for pattern recognition, clearly showing
that the proposed MS profiling method was able to classify Oriental Beauty tea
into different grades. The component mass ions primarily responsible for the
separation were selected with high loading strength in the PCA for subsequent
identification with tandem mass (MS/MS). Caffeine, citrate and salicylate were
verified, whereas certain other compounds remained ambiguous. Regression
analysis considering caffeine, citrate and salicylate showed a linear
relationship between the prices of the Oriental Beauty tea with an adjusted R2
of 0.84. If all the selected marker ions (in addition to caffeine, citrate and
salicylate) could have been identified and incorporated into regression
analysis, a stronger relationship could have been confirmed. These results
suggest that metabolomics can facilitate the determination of real markers in
the quality control of Oriental Beauty tea, and may lead to the further
application of metabolomics in other food quality controls.
关键词：Dongfang Meiren; High Performance Liquid Chromatography; Mass Spectrometry; Metabolic Profiling; Metabolomics; Oriental Beauty; Quality; Tea
