摘要:In Croatia, the Balkan loach, Cobitis elongata inhabits the Sava River basin. We found significant morphological differences between specimens inhabiting the Kupa River and those from its right tributary the Petrinjčica River. Results of ANOVA analysis were statistically significant for 17 measurements and all statistically significant parameters were considerably higher at the Petrinjčica River sites. These differences are probably caused by the spatial isolation of these two populations due to artificial and natural barriers on the Petrinjčica River and different ecological features of habitats in the Kupa and the Petrinjčica River.