文章基本信息

标题：Cultivation of CCO cells on non-porous microcarriers in low-serum conditions.
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作者：Slivac, Igor ; Radošević, Kristina ; Dukić, Bogdanka 等
期刊名称：Croatian Journal of Food Technology, Biotechnology and Nutrition
印刷版ISSN：1847-3423
电子版ISSN：1847-7461
出版年度：2014
卷号：9
期号：1-2
页码：43-47
语种：Croatian
出版社：Hrvatsko društvo prehrambenih tehnologa, biotehnologa i nutricionista
摘要：In this study Cytodex 1 microcarriers were used for Channel Catfish Ovary (CCO) cell cultivation and biomass production in Spinner-flask. Cell attachment, cell number and glucose consumption were determined during 312 h cultivation in two media with 5% and 2% of serum. CCO cell cultivation in medium with lower serum concentration resulted in higher cell yield and specific growth rate when compared to standard growth medium. Obtained results showed that tested UltraCulture medium was good choice when cultivation of CCO cells in reduced serum conditions is demanded.
关键词：CCO cells; Cell-spin; cell yield; microcarriers; serum;