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  • 标题:Stress Effects on Virulence-related Genes Expression in Enterococcus faecalis from
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  • 作者:Ming Lei ; Xianjun Dai ; Mingqi Liu
  • 期刊名称:Advance Journal of Food Science and Technology
  • 印刷版ISSN:2042-4868
  • 电子版ISSN:2042-4876
  • 出版年度:2014
  • 卷号:6
  • 期号:4
  • 页码:547-551
  • 出版社:MAXWELL Science Publication
  • 摘要:Enterococcus faecalis are ubiquitous micro-organisms which are widely used in fermented food or probiotic industry. However, they are also associated with nosocomial infections and rank among the top three nosocomial bacterial pathogens. Some virulence factors were proposed which could strongly enhance their pathogenicity. In our study, three virulence-related genes ace, gelE and efaA were found in E. faecalis SL22 which was isolated from raw milk. The expression of these genes was examined in response to different stress factors like NaCl, pH and gentamycin sulfate by using real-time PCR. Significant differences in expression level were influenced by the environment factors, which suggests that these stress factors should be taken into consideration during the selection of enterococci for use in foods or as probiotics.
  • 关键词:E. faecalis ; expression level ; stress factor ; virulence-related genes ; ; ;
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