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  • 标题:D2-like receptor activation does not initiate a brain docosahexaenoic acid signal in unanesthetized rats
  • 本地全文:下载
  • 作者:Ameer Y Taha ; Lisa Chang ; Mei Chen
  • 期刊名称:BMC Neuroscience
  • 印刷版ISSN:1471-2202
  • 电子版ISSN:1471-2202
  • 出版年度:2014
  • 卷号:15
  • 期号:1
  • 页码:1
  • DOI:10.1186/1471-2202-15-113
  • 语种:English
  • 出版社:BioMed Central
  • 摘要:Background The polyunsaturated fatty acid, docosahexaenoic acid (DHA), participates in neurotransmission involving activation of calcium-independent phospholipase A2 (iPLA2), which is coupled to muscarinic, cholinergic and serotonergic neuroreceptors. Drug induced activation of iPLA2 can be measured in vivo with quantitative autoradiography using 14C-DHA as a probe. The present study used this approach to address whether a DHA signal is produced following dompaminergic (D)2-like receptor activation with quinpirole in rat brain. Unanesthetized rats were infused intravenously with 14C-DHA one minute after saline or quinpirole infusion, and serial blood samples were collected over a 20-minute period to obtain plasma. The animals were euthanized with sodium pentobarbital and their brains excised, coronally dissected and subjected to quantitative autoradiography to derive the regional incorporation coefficient, k*, a marker of DHA signaling. Plasma labeled and unlabeled unesterified DHA concentrations were measured. Results The incorporation coefficient (k*) for DHA did not differ significantly between quinpirole-treated and control rats in any of 81 identified brain regions. Plasma labeled DHA concentration over the 20-minute collection period (input function) and unlabeled unesterified DHA concentration did not differ significantly between the two groups. Conclusion These findings demonstrate that D2-like receptor initiated signaling does not involve DHA as a second messenger, and likely does not involve iPLA2 activation.
  • 关键词:Dopamine ; Quinpirole ; Neurotransmission ; Docosahexaenoic acid ; Release ; Signaling ; Metabolism ; Intracellular calcium ; Calcium-independent phospholipase A 2 (iPLA 2 ) ; Calcium-dependent phospholipase A 2 (cPLA 2 ) ; Arachidonic acid
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