This study aims to examine the effect of ozone application on quality characteristics of different types of flour samples and their dough and bread samples. For this purpose, ozone was applied to the half of the three different flour samples (Type 550, 650 and 850) by an ozone generator. The other half of the flour samples were designated as the control group with no ozone application. Wet gluten, gluten index and color values (L*, a* and b*) were undertaken as the parameters in determination of flour quality. The Farinograph and Extensograph characteristics were taken into consideration in evaluation of doughs which were made from those flours and then, the qualitative characteristics of breads made from these flours. According to the obtained data, while the wet gluten and the L* values were increased, b* values were decreased by the ozone application. Besides, it increased the whiteness of the flour. It is determined that the ozone application had no significant effect on the rheological properties of the dough but, the energy values of the dough were significantly affected. It is also found that the ozone application had a significant positive effect on the bread volumes and specific volumes, especially for the breads made from Type 550 flours. In general, the ozone application had positive effects on the textural structure and the whiteness of the crumb.