摘要：In this study, the effects of UV-C irradiation on secondary metabolite accumulation in callus cultures of “Gamay” ( Vitis vinifera L.) grape cultivar were investigated. Calli were obtained from petiole on B5 media added 0.5 mg/L BAP (benzyl amino purin), 0.5 mg/L IAA (indole acetic acid), 3% sucrose and 0.8% agar agar. UV-C was applied onto 12 days old calli at 10 and 15 cm distances from the source, for 10, 15 and 20 min. After treatment, calli were cultured in dark conditions at 25 oC and sampled after 0, 24 and 48 hours. Total phenolic compound, total flavanols, total flavonols, anthocyanin and carotenoid contents were determined by spectrophotometrically. All criteria were changed greatly depending on the combination of irradiation distance from UV-C source, treatment period and sampling time. It was determined that UV-C irradiation had remarkable promoting effects on the accumulation of phenolics and carotenoid in the calli of Gamay grape cultivar.
