首页    期刊浏览 2024年09月20日 星期五
登录注册

文章基本信息

  • 标题:Use of Molecular Based Methods for Identification of Foodborne Yeasts
  • 本地全文:下载
  • 作者:Seda Karasu Yalçın ; Şule Şenses Ergül ; Z. Yeşim Özbaş
  • 期刊名称:Gida : Journal of Food
  • 印刷版ISSN:1300-3070
  • 电子版ISSN:1309-6273
  • 出版年度:2010
  • 卷号:35
  • 期号:5
  • 出版社:Association of Food Technology, Turkey
  • 摘要:Yeasts, which are very important microorganisms for food industry, can contribute to both production and spoilage of various foods. It is known that culturing techniques used for yeast identification are time consuming and have low accuracy and reproducibility. For this reason, studies concerning the use of DNA based techniques giving fast and accurate identification results, have been increased in recent years. Re¬striction Fragment Length Polymorphism (RFLP), depending on cutting of the Polymerase Chain Reaction (PCR)-amplified DNA fragments by restriction enzymes, is given as one of the most common methods used for this purpose. Genotyping of the yeast strains of a species by molecular methods can be performed by various techniques such as Randomly Amplified Polymorphic DNA (RAPD). Identification and geno-typing of foodborne yeasts by molecular methods have major importance in monitoring fermentations, determination of contamination sources, preventing of food spoilage and determining of industrially im¬portant yeast strains.
  • 关键词:Food; Yeast; Molecular identification; Genotyping; RFLP-PCR; RAPD-PCR
国家哲学社会科学文献中心版权所有