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  • 标题:A Rapid Method Intended for the Determination of Salmonella spp. From Chicken Meats
  • 本地全文:下载
  • 作者:Birce Mercanoğlu Taban ; S. Aykut Aytaç
  • 期刊名称:Gida : Journal of Food
  • 印刷版ISSN:1300-3070
  • 电子版ISSN:1309-6273
  • 出版年度:2008
  • 卷号:33
  • 期号:5
  • 出版社:Association of Food Technology, Turkey
  • 摘要:Since contaminated chicken meats have been the principal foodborne source of the contamination of Salmonella to humanbeings and cultural detection methods are time-consuming; in this study, which had been based on the improvement of a Salmonella spp. detection method that can give rapid and high sensitivity result in chicken meats, firstly chicken meat samples, in which no Salmonella contamination had been determined by using ISO 6579 reference method, were inoculated with Salmonella Enteritidis culture and after the 16-hour non selective pre-enrichment stage, approximately 1 or 10 cfu /25 gram chicken meat levels of target bacteria determination was ensured in totally 20 hours by using immunomagnetic separation-polymerase chain reaction ( IMS -PCR) method.
  • 关键词:IMS ; PZR ; Salmonella ; chicken meat
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