Triglyceride structures of four different vegetable oils (soybean, sunflower, corn and olive oils) were determined by reversed phase high performance liquid chromatography (RP-HPLC) equipped with evaporative light scattering detector (ELSD) and the results were compared with those obtained by enzymatic method. According to the results, there are not considerable differences between the results obtained by both methods. However, some glyceride peaks and isomers were not able to be separated by RP-HPLC and enzymatic method was not found sensitive to some fatty acids.