摘要：Separation of flavonoids by the combining of descending paper chromatography and High Pressure Liquid Chromatography (HPLC) techniques were investigated. For paper chromatography Whatman 3MM chromatography paper and BAW solvent were used and 7 bands obtained. Flavonoids on the bands were eluted with MAW solvent and injected to the HPLC column. Results revealed that 15 peaks can be obtained purely. This method could be used for purification of different kinds of complex compounds.
