标题:Caractérisation phénotypique et génotypique des bactéries lactiques isolées des farines de blé d’origine marocaine (Phenotypic and genotypic characterization of lactic acid bacteria isolated from wheat flour from Morocco),
期刊名称:Journal of Materials and Environmental Science
印刷版ISSN:2028-2508
出版年度:2014
卷号:5
期号:4
页码:1125-1132
出版社:University of Mohammed Premier Oujda
摘要:A total of 60 samples of wheat flours sampled from different Moroccan regions have been investigated for their lactic acid bacteria microbial diversity. Among 60 lactic acid bacteria isolates, 30 strains, have been isolated and identified using biochemical and genotypic methods (PCR) and seq uencing of the gene coding for phenylalanine 16s rRN A. It has been shown that reliable identification of the isolates required the use of molecular techniq ues and bioinformatic so ftware with large databases. The results showed that lactic acid bacteria isolated from Moroccan flour belonged to the genera Pediococcus, Enterococcus, Lactobacillus, Lactococcus and Weissella, with the dominance of Pediococcus (63%) compared to the total number of strains, follo wed b y Enterococcus with a frequency o f 27% and finally Weissella, Lactococcus and Lactobacillus with a frequency of 10%