摘要：The aim of this study was to assess biofilm formation by some pathogenic strains of E. coli and Salmonella sp. using glass and cement coupons, varying nutrient-culture media and two incubation temperatures (refrigeration (11°C) and room (28±2°C)). The Crystal Violet Binding Assay (CVBA) and Bacteria Cell Enumeration (BCE) methods were used to quantify E. coli and Salmonella biofilms in 5 days while Scanning Electron Microscopy (SEM) was utilized in viewing the formed biofilms. Though, it was observed that all tested organisms did not grow at the same rate, generally, Salmonella sp. produced higher biofilms than E. coli O157:H7 strains. More biofilms were formed on cement surfaces than glass and was confirmed with SEM. Biofilms developed on the coupons were higher at room temperature and in most cases higher in media with increased nutrient concentrations. With these results, it can be deduced that there is a higher presence of biofilms formed by pathogenic organisms in our food processing plants where the principles of hygiene are not held in high esteem. Therefore, regular evaluation of operation cleanliness in the food processing environments is highly necessary in order to control these pathogens and their biofilms.
