摘要：The use of probiotic affects the aquatic animals immune response and growth performance. This study aimed to isolate bacteria from freshwater lobsters intestines and observe in vitro the isolates ability in utilizing alginate from brown alga ( Sargassum sp.) as a prebiotic. The bacteria was isolated from the freshwater lobster ( Cerax quadricarinatus ) intestines and the water of the lobster habitat. The lobster were collected from four stations i.e., Makassar, Bantimurung, Maros and Mandalle. The results of the study showed that after being soaked in a physiological solution with a pH of 2, there were 30 species of bacterial isolates discovered. The antagonistic test of inhibition zone against the pathogenic bacteria Aeromonas hydrophila showed the isolates of MDL7, MKS4 and MKS3 might inhibit the growth of A. hydrophila . While co-culture antagonistic test revealed that the isolates MDL1, MDL3, MDL6, BTL5, MRS4 and MRS5 could inhibit the growth of the pathogenic bacteria A. hydrophila . In vitro culture of alginate as prebiotic with the four best isolates as probiotic candidates, namely MDL7, MKS4, BTL5 and MRS5 demonstrated that the probiotic bacteria could grow and utilized prebiotic optimally, especially the MRS5 and BTL5 probiotic bacteria. Therefore, alginate as a prebiotic and MRS5 and BTL5 as probiotic bacteria have a great potential to be a symbiotic.
