摘要：An experiment was conducted to study the effects of Thyme Methanol Extract (TME), on rumen methanogenesis in vitro . Different levels (0, 0.25 and 0.5 mL) of TME were incubated for 2, 4, 12, 16, 24, 48, 72 and 96 h in diluted ruminal fluid with a 100% forage diet (alfalfa) to evaluate their effects on rumen methane production. This experiment was carried out based on Complete Random Design (CRD) with three treatments and three replicates. Treatment A is 0 mL of TME and diet (control), Treatment B is 0.25 mL of TME and diet and Treatment C is 0.5 mL TME and diet. Result indicated that the methanol extract of thyme (0.25 and 0.50 mL) reduced methane production in all of the incubation times without 2 h (p<0.05).
