摘要：Biological treatments for degradation of chlorophenols are one of the promising treatment techniques as they are relatively less expensive and result in complete mineralisation. Degradation experiments were conducted using Pseudomonas putida (NCIM sp. 2650) under aerobic conditions in two reactors, connected in series. The effectiveness of Pseudomonas putida to degrade synthetic 4-chlorophenols (4-CP) as well as industrial effluent was tested along with glucose as a carbon source . The synthetic 4-CP initial concentrations were varied from 60, 80 and 100 ppm and it was observed that an equilibrium degradation of 66, 68 and 93% was achieved for reactor 1 and 95, 97 and 99% degradation for reactor 2 at 60, 80 and 100 ppm of 4-CP initial concentrations, respectively. The reactor 2 effluent was also analyzed for Biological Oxygen Demand (BOD3) and Chemical Oxygen Demand (COD) and an average BOD3 reduction of 95% and COD reduction of 94% was achieved for all three initial 4-CP concentrations. Equilibrium degradation of 69% and 98% was achieved for reactor 1 and 2 for industrial effluent with a BOD3 reduction, 96% and COD reduction, 91% showing that Pseudomonas putida possessed the strong capacity to degrade 4-CP.
