期刊名称：Journal of Environmental Science and Technology
印刷版ISSN：1994-7887
电子版ISSN：2077-2181
出版年度：2014
卷号：7
期号：1
页码：76-83
DOI：10.3923/jest.2014.76.83
出版社：Asian Network for Scientific Information
摘要：Increasing Carbon Dioxide (CO2) in the atmosphere has aggravated the threats of global warming and global diversity loss. To decrease CO2 emission, various physical and chemical based strategies are used to store and sequester CO2. The biomimmetic approach is one of the available harmless, robust and eco-friendly approach but still less evaluated. The present study reports the role of microbial intracellular carbonic anhydrase in the formation of calcite and sequestration of CO2 by bacteria. Bacterial carbonic anhydrase was screened from Bacillus altitudinis , isolated from the mangrove forest of the western coastline of India. The isolate was able to grow optimally at 2.5 % CO2 saturated environment. The enzyme was found to catalyze the reaction in a wide range of pH and temperature with an optimum at 7.5 pH and 30°C temperature. The enzyme was reported to accelerate the hydration of CO2 in 50 mM ZnCl2. Furthermore the enzyme remained active and shown the remarkable activity with sulphanilamide inhibitor. in vitro calcite encrust formation activity was tested using growth media supplemented with CaCl2. Product of sequestration, Calcite, can be used as raw material for building construction.
