Recently fibrin glue, a hemostatic or adhesive material has been used worldwide in surgical procedures. There have been only limited numbers of reports on anaphylaxis following the topical use of fibrin glue. Here, we experienced an anaphylactoid reaction during transsphenoidal removal of pituitary tumor after local application of fibrin glue including aprotinin. Unexpected hypotension and erythematous eruptions on whole body developed suddenly after packing of it at operation field. Treatment with aggressive volume replacement with vasoactive agents and steroids were done immediately on the diagnosis of anaphylactoid reaction and patient recovered without any complications.