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  • 标题:Effect of High Glucose on the Oxidative Stress in Trabecular Meshwork Cells
  • 本地全文:下载
  • 作者:Kim, Jae Woo ; Bae, Chang Beum ; Lee, Jeong Hun
  • 期刊名称:Journal of the Korean Ophthalmological Society
  • 印刷版ISSN:0378-6471
  • 出版年度:2009
  • 卷号:50
  • 期号:10
  • 页码:1563-1568
  • DOI:10.3341/jkos.2009.50.10.1563
  • 语种:Korean
  • 出版社:The Korean Ophthalmological Society
  • 摘要:Purpose

    To investigate the effect of high glucose (HG) on the oxidative stress in cultured human trabecular meshwork cells (HTMC).

    Methods

    Primarily cultured HTMC were exposed to low glucose (5 mM) and HG (25 mM) for 7 days. Additionally, 1 mM L-arginine, 5 mM DAHP, 10 µg/ml insulin, 100 µM L-ascorbic acid, 10, and 100 µM sepiapterin were co-exposed. The cellular survival and nitric oxide (NO) production were assessed by MTT assay and Griess assay, respectively. Superoxide production was measured by modified cytochrome c assay.

    Results

    HG did not affect the survival of cultured HTMC significantly. HG decreased NO production. Co-exposed DAHP decreased but DAHP and insulin increased NO production. In addition, HG increased superoxide production, which was decreased by insulin, L-ascorbic acid, and sepiapterin.

    Conclusions

    HG decreased NO production accompanied with increased superoxide production in HTMC. Thus HG induces oxidative stress in HTMC and may cause cellular dysfunction and damage of the trabecular meshwork.

  • 关键词:Glucose; Nitric Oxide; Oxidative stress; Superoxide; Trabecular meshwork cells
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