文章基本信息
- 标题：Role of Nitric Oxide in the Proliferative and Migratory Effect of Triamcinolone in RPE Cells
- 本地全文：下载
- 作者：Kim, Jae Woo ; Lee, Jae Hyung ; Lee, Seung Hee 等
- 期刊名称：Journal of the Korean Ophthalmological Society
- 印刷版ISSN：0378-6471
- 出版年度：2010
- 卷号：51
- 期号：1
- 页码：120-125
- DOI：10.3341/jkos.2010.51.1.120
- 语种：Korean
- 出版社：The Korean Ophthalmological Society
- 摘要：Purpose
To investigate the role of nitric oxide (NO) on the proliferative and migratory effects of triamcinolone acetonide (TA) in retinal pigment epithelial cells.
MethodsAfter exposure to 10 nM, 1 µM, or 100 µM TA for four days, with or without co-exposure of antioxidant N-acetylcyteine, the proliferation and nitrite production of ARPE19 cells were assessed with MTT and Griess assays, respectively. Additionally, a cell migration assay was performed.
ResultsCellular survival increased after exposure to TA at low concentration but decreased at high concentration. TA decreased the production of NO and cellular migration significantly, and these effects were abolished by N-acetylcysteine.
ConclusionsTA showed a biphasic response on the proliferation and decreased cellular migration in ARPE19 cells, which may be mediated by nitric oxide.
- 关键词：ARPE19; Migration; Nitric Oxide; Triamcinolone acetonide
