文章基本信息
- 标题：Effect of Glucose on the Production of Reactive Oxygen Species in Retinal Pigment Epithelial Cells
- 本地全文：下载
- 作者：Lee, Jae Hyung ; Kim, Jae Woo
- 期刊名称：Journal of the Korean Ophthalmological Society
- 印刷版ISSN：0378-6471
- 出版年度：2010
- 卷号：51
- 期号：2
- 页码：276-281
- DOI：10.3341/jkos.2010.51.2.276
- 语种：Korean
- 出版社：The Korean Ophthalmological Society
- 摘要：Purpose
To investigate the effect of high glucose (HG) on the production of reactive oxygen species (ROS) in retinal pigment epithelial (RPE) cells.
MethodsARPE-19 cells were exposed to low glucose (5 mM) and high glucose (HG, 25 mM) for three days. Additionally, 50 µM N-acetyl cysteine (NAC) and 100 µM L-arginine were co-exposed. Productions of nitric oxide (NO), ROS, and superoxide were determined by Griess assay, DCFH-DA assay, and modified cytochrome c assay, respectively.
ResultsHG increased production of NO, ROS, and superoxide. HG-induced cells increased production of superoxide and ROS but were suppressed by NAC and L-arginine (substrate for NO production) as an antioxidant.
ConclusionsHG increased ROS production in RPE cells. Thus, HG may cause cellular dysfunction and damage by inducing oxidative stress in RPE cells.
- 关键词：High glucose; Oxidative stress; Reactive Oxygen Species; Retinal pigment epithelial cells
