文章基本信息
- 标题：Effect of High Glucose on the Production of Reactive Oxygen Species in Trabecular Meshwork Cells
- 本地全文：下载
- 作者：Bae, Chang Beum ; Kim, Jae Woo
- 期刊名称：Journal of the Korean Ophthalmological Society
- 印刷版ISSN：0378-6471
- 出版年度：2010
- 卷号：51
- 期号：3
- 页码：418-422
- DOI：10.3341/jkos.2010.51.3.418
- 语种：Korean
- 出版社：The Korean Ophthalmological Society
- 摘要：Purpose
To investigate the effect of high glucose concentration on the production of reactive oxygen species in cultured human trabecular meshwork cells (HTMCs).
MethodsPrimarily cultured HTMCs were exposed to low glucose (5 mM) and high glucose (22 mM) concentrations, respectively, for seven days. Cellular survival, as well as nitric oxide (NO) and hydrogen peroxide production, were assessed by measured MTT assay, Griess assay, and o-Dianisidine dihydrochloride assay, respectively. Some cells were co-exposed to N-acetyl cysteine (NAC) to assess the effect of this antioxidant.
ResultsHigh glucose concentration increased the survival of cultured HTMCs significantly, with no effect from NAC. High glucose concentration increased the production of NO and hydrogen peroxide, which were abolished by co-exposure with NAC.
ConclusionsHigh glucose concentration increases the production of NO and hydrogen peroxide, which can be abolished by antioxidant in trabecular meshwork cells.
- 关键词：High glucose; hydrogen peroxide; Nitric Oxide; Reactive Oxygen Species; Trabecular meshwork cells
