文章基本信息
- 标题：Effect of High Glucose on the Production of Reactive Oxygen Species in R28 Cells
- 本地全文：下载
- 作者：Lee, Jong Min ; Kim, Jae Woo
- 期刊名称：Journal of the Korean Ophthalmological Society
- 印刷版ISSN：0378-6471
- 出版年度：2010
- 卷号：51
- 期号：5
- 页码：746-750
- DOI：10.3341/jkos.2010.51.5.746
- 语种：Korean
- 出版社：The Korean Ophthalmological Society
- 摘要：Purpose
To investigate the effect of high glucose (HG) on the production of reactive oxygen species (ROS) in retinal precursor R28 cells.
MethodsR28 cells were incubated with low glucose (5 mM) or HG (25 mM) for two days. Additionally, the cells were co-exposed to 50 µM N-acetyl cysteine or 100 µM L-arginine. Production of nitric oxide (NO), ROS, and superoxide were assessed by Griess assay, DCFH-DA assay, and modified cytochrome c assay, respectively.
ResultsHG increased the production of NO, ROS, and superoxide, which were abolished by antioxidants NAC and L-arginine (a substrate for NO production).
ConclusionsHG increased ROS production in R28 cells. Thus, HG may cause cellular dysfunction and damage by inducing oxidative stress in retinal ganglion cells.
- 关键词：High glucose; Oxidative stress; Reactive Oxygen Species; Retinal ganglion cells
