文章基本信息

标题：Effect of Nitric Oxide on the Trabecular Meshwork Cell-mediated Contraction of Collagen Gels
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作者：Kim, Jae Woo ; Chang, Woo Seok ; Lee, Su Yoon 等
期刊名称：Journal of the Korean Ophthalmological Society
印刷版ISSN：0378-6471
出版年度：2007
卷号：48
期号：11
页码：1548-1553
DOI：10.3341/jkos.2007.48.11.1548
语种：Korean
出版社：The Korean Ophthalmological Society
摘要：Purpose
To investigate the effect of nitric oxide (NO) on the contraction of cultured human trabecular meshwork cells (HTMCs).
Methods
After embedding them into collagen gels, primarily cultured HTMCs were exposed to NO donors, such as sodium nitroprusside (SNP) or S-Nitroso-N-acetylpenicillamine (SNAP), for 1 week at various concentrations, and the contraction of the collagen gels was measured. Cellular survival and NO production were measured with MTT assay and Griess assay, respectively.
Results
Though SNP and SNAP did not significantly affect cellular survival, they markedly enhanced NO production. Both sodium nitroprusside and SNAP inhibited the contraction of collagen gels by about 10% in dose and time-dependent manners (p<0.05).
Conclusions
NO donors inhibited the contraction of collagen gels in vitro. Thus, NO donors may relax trabecular meshwork and enhance trabecular outflow.
关键词：Collagen gel; Contraction; Nitric Oxide; Trabecular Meshwork