文章基本信息
- 标题：Effect of Hydrogen Peroxide-induced Oxidative Stress on the Senescence of Trabecular Meshwork Cells
- 本地全文：下载
- 作者：Kim, Jae Woo ; Kim, Sin Hoo ; Lee, Jae Hyung 等
- 期刊名称：Journal of the Korean Ophthalmological Society
- 印刷版ISSN：0378-6471
- 出版年度：2008
- 卷号：49
- 期号：10
- 页码：1665-1670
- DOI：10.3341/jkos.2008.49.10.1665
- 语种：Korean
- 出版社：The Korean Ophthalmological Society
- 摘要：Purpose
To investigate the effects of oxidative stress on the senescence of trabecular meshwork (TM) cells and the effect of L-ascorbic acid (LAA) against oxidative stress-induced senescence.
MethodsPrimary cultured human TM cells were exposed to 0.05 or 0.1 mM hydrogen peroxide for 30 minutes and incubated for 1 week with or without co-exposure of LAA. Cellular survival, nitrite production, and senescence were assessed with MTT, Griess, and SA-β-gal assays, respectively.
ResultsHydrogen peroxide decreased cellular survival and NO production accompanied increased cellular senescence. LAA did not prevent hydrogen peroxide-induced senescence.
ConclusionsOxidative stress-induced senescence of TM cells may be related to the dysfunction of trabecular meshwork in glaucoma.
- 关键词：Ascorbic Acid; hydrogen peroxide; Oxidative stress; Senescence; Trabecular meshwork cells
