文章基本信息
- 标题：Effect of Erythropoietin on the Production of Nitric Oxide in Trabecular Meshwork Cells
- 本地全文：下载
- 作者：Lee, Seung Hee ; Kim, Jae Woo
- 期刊名称：Journal of the Korean Ophthalmological Society
- 印刷版ISSN：0378-6471
- 出版年度：2011
- 卷号：52
- 期号：12
- 页码：1514-1518
- DOI：10.3341/jkos.2011.52.12.1514
- 语种：Korean
- 出版社：The Korean Ophthalmological Society
- 摘要：Purpose
To investigate the effects of erythropoietin (EPO) on the production of nitric oxide in cultured human trabecular meshwork cells (HTMC).
MethodsPrimarily cultured HTMC were exposed to 0, 0.5, and 1.0 U/ml EPO using serum-deprived media for 2 days. Production of nitric oxide and cellular survival were assessed with Griess assay and MTT assay, respectively. Expression of EPO mRNA receptor and activity of eNOS were assessed with RT-PCR.
ResultsEPO did not affect the survival of HTMC ( p > 0.05). EPO increased the production of nitric oxide ( p < 0.05) accompanied with increased eNOS activity.
ConclusionsEPO had no effect on the cellular survival under serum-deprived conditions. EPO increases the production of nitric oxide by increasing eNOS activity.
- 关键词：Erythropoietin; Nitric Oxide; Trabecular meshwork cells
