文章基本信息
- 标题：Effect of Ascorbic Acid Against the Oxidative Stress-Induced Cellular Senescence in Trabecular Meshwork Cells
- 本地全文：下载
- 作者：Kim, Jae Woo ; Kang, Sun Hee ; Lee, Keun Woo 等
- 期刊名称：Journal of the Korean Ophthalmological Society
- 印刷版ISSN：0378-6471
- 出版年度：2013
- 卷号：54
- 期号：3
- 页码：490-495
- DOI：10.3341/jkos.2013.54.3.490
- 语种：Korean
- 出版社：The Korean Ophthalmological Society
- 摘要：Purpose
To investigate the effect of L-ascorbic acid (LAA) on oxidative-stress-induced cellular senescence in trabecular meshwork (TM) cells.
MethodsPrimarily cultured human TM cells were exposed to 0, 10, or 100 µM hydrogen peroxide for 7 days with or without co-exposure of LAA. Cellular survival and nitrite production were assessed with MTT and Griess assays, respectively. SA-β-gal staining was performed to quantify cellular senescence.
ResultsHydrogen peroxide decreased cellular survival, accompanied by decreased nitric oxide (NO) production. These decreases of cellular survival and NO production were abolished by co-exposure of 100 µM LAA. Analysis of SA-β-gal staining revealed that LAA inhibited hydrogen peroxide-induced cellular senescence by 6.8% ( p < 0.05).
ConclusionsLAA may have a protective effect against the oxidative-stress-induced cellular senescence in TM cells.
- 关键词：Ascorbic Acid; Oxidative stress; Senescence; Trabecular meshwork cells
