文章基本信息
- 标题：Effect of Bevacizumab and Ranibizumab on the Expression of eNOS in Trabecular Meshwork Cells
- 本地全文：下载
- 作者：Kim, Se Eun ; Kim, Jae Woo
- 期刊名称：Journal of the Korean Ophthalmological Society
- 印刷版ISSN：0378-6471
- 出版年度：2014
- 卷号：55
- 期号：8
- 页码：1208-1212
- DOI：10.3341/jkos.2014.55.8.1208
- 语种：Korean
- 出版社：The Korean Ophthalmological Society
- 摘要：Purpose
To compare the effects of bevacizumab and ranibizumab on the expression of eNOS in cultured human trabecular meshwork cells (HTMC).
MethodsPrimarily cultured HTMC were exposed to 0.5 mg/mL bevacizumab and ranibizumab using 1% serum-containing media for 30 minutes. Expression of eNOS mRNA was assessed with RT-PCR. Additionally, after exposure to 20 ng/mL of vascular endothelial growth factor (VEGF) and 0.5 mg/mL bevacizumab and ranibizumab, the production of nitric oxide was assessed with Griess assay.
ResultsVEGF increased the production of nitric oxide in HTMC. Bevacizumab and ranibizumab decreased the expression of eNOS mRNA and production of nitric oxide ( p < 0.05) in HTMC. The decrease in eNOS mRNA expression and production of nitric oxide was not significant between bevacizumab and ranibizumab ( p > 0.05).
ConclusionsIn HTMC, both bevacizumab and ranibizumab decreased the expression of eNOS mRNA with no significant difference observed between the two drugs.
- 关键词：Bevacizumab; eNOS; Nitric Oxide; Ranibizumab; Trabecular Meshwork
