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  • 标题:Induction of apoptosis by a hexane extract of aged black garlic in the human leukemic U937 cells
  • 本地全文:下载
  • 作者:Park, Cheol ; Park, Sejin ; Chung, Yoon Ho
  • 期刊名称:Nutrition Research and Practice
  • 印刷版ISSN:1976-1457
  • 出版年度:2014
  • 卷号:8
  • 期号:2
  • 页码:132-137
  • DOI:10.4162/nrp.2014.8.2.132
  • 语种:English
  • 出版社:KoreaMed Synapse
  • 摘要:BACKGROUND/OBJECTIVES

    In this study, the apoptogenic activity and mechanisms of cell death induced by hexane extract of aged black garlic (HEABG) were investigated in human leukemic U937 cells.

    MATERIALS/METHODS

    Cytotoxicity was evaluated by MTT (3-(4, 5-dimethyl-thiazol-2-yl)-2, 5-diphenyl tetrazoliumbromide) assay. Apoptosis was detected using 4,6-diamidino-2-phenyllindile (DAPI) staining, agarose gel electrophoresis and flow cytometry. The protein levels were determined by Western blot analysis. Caspase activity was measured using a colorimetric assay.

    RESULTS

    Exposure to HEABG was found to result in a concentration- and time-dependent growth inhibition by induction of apoptosis, which was associated with an up-regulation of death receptor 4 and Fas legend, and an increase in the ratio of Bax/Bcl-2 protein expression. Apoptosis-inducing concentrations of HEABG induced the activation of caspase-9, an initiator caspase of the mitochodrial mediated intrinsic pathway, and caspase-3, accompanied by proteolytic degradation of poly(ADP-ribose)-polymerase. HEABG also induced apoptosis via a death receptor mediated extrinsic pathway by caspase-8 activation, resulting in the truncation of Bid, and suggesting the existence of cross-talk between the extrinsic and intrinsic pathways. However, pre-treatment of U937 cells with the caspase-3 inhibitor, z-DEVD-fmk, significantly blocked the HEABG-induced apoptosis of these cells, and increased the survival rate of HEABG-treated cells, confirming that HEABG-induced apoptosis is mediated through activation of caspase cascade.

    CONCLUSIONS

    Based on the overall results, we suggest that HEABG reduces leukemic cell growth by inducing caspase-dependent apoptosis through both intrinsic and extrinsic pathways, implying its potential therapeutic value in the treatment of leukemia.

  • 关键词:Aged black garlic; U937 cells; Apoptosis; Caspase
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