文章基本信息
- 标题：Induction of IL-8 and reactive oxygen species in periodontal ligament cells by Aggregatibacter actinomycetemcomitans
- 本地全文：下载
- 作者：Lee, Yang-Sin ; Park, Hong Gyu ; Kim, Sung Whan 等
- 期刊名称：The Journal of the Korean Academy of Periodontology
- 印刷版ISSN：0250-3352
- 出版年度：2009
- 卷号：39
- 期号：3
- 页码：331-337
- DOI：10.5051/jkape.2009.39.3.331
- 语种：Korean
- 出版社：Korean Academy of Periodontology
- 摘要：Purpose
Interleukin (IL)-8 is one of pro-inflammatory cytokines. Reactive oxygen species (ROS) are reduced metabolites of O2. Aggregatibacter actinomycetemcomitans is one of representative periodontopathogens. To investigate the role of A. actinomycetemcomitans in IL-8 expression of periodontal ligament (PDL) cells, we estimated the production of IL-8 and ROS in A. actinomycetemcomitans treated PDL cells.
MethodsThe IL-8 production was determined by enzyme-linked immunosorbent assay. The ROS production was estimated using H2DCFDA and FACS.
ResultsA. actinomycetemcomitans increased the production of IL-8 and ROS at 10, 100, and 500 multiplicity of infection. N-acetylcysteine, an antioxidant of ROS, down-regulated the production of IL-8 induced by A. actinomycetemcomitans .
ConclusionsThese results suggest that A. actinomycetemcomitans induces IL-8 production and ROS may act as a mediator in this process.
- 关键词：Aggregatibacter actinomycetemcomitans; Interleukin-8; Periodontal ligament; Reactive Oxygen Species
