文章基本信息

标题：Response of fetal rat calvarial cells on mineral trioxide aggregate after IL-1β stimulation
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作者：Lee, Sool-Heon ; Park, Ji-Il ; Kim, Young-Joon 等
期刊名称：The Journal of the Korean Academy of Periodontology
印刷版ISSN：0250-3352
出版年度：2009
卷号：39
期号：3
页码：359-365
DOI：10.5051/jkape.2009.39.3.359
语种：Korean
出版社：Korean Academy of Periodontology
摘要：Purpose
The purpose of this study was to investigate the ability of Mineral trioxide aggregate(MTA) to support osteoclastic differentiation from fetal rat calvarial cell.
Methods
In this study, response of IL-6, RANKL, and OPG in fetal rat calvarial cells stimulated with IL-1β on MTA was evaluated by ELISA and RT-PCR.
Results
The results were as follows; there was no significant difference between glass and MTA at 5days. In ELISA analysis, Glass group and MTA group showed similar IL-6 expression, Glass+IL-1β group and MTA+IL-1β group showed similar IL-6 expression. In RT-PCR analysis, Glass group and MTA group showed similar IL-6, RANKL, OPG mRNA expression, MTA+IL-1β group and Glass+IL-1β group showed 3 fold increase of IL-6 and RNAKL mRNA expression when compared with MTA group. All groups showed similar OPG mRNA expression.
Conclusions
MTA does not suppress cell proliferation and increase the proinflammatory cytokine that induce osteoclastogenesis. Thus, MTA is biocompatible material that could be used in various clinical conditions.
关键词：Biocompatible materials; Cytokines; ELISA; mineral trioxide aggregate