The aim of this study was to evaluate the surface properties and biological response of an anodized titanium surface by cell proliferation and alkaline phosphatase activity analysis.

Commercial pure titanium (Ti) disks were prepared. The samples were divided into an untreated machined Ti group and anodized Ti group. The anodization of cp-Ti was formed using a constant voltage of 270 V for 60 seconds. The surface properties were evaluated using scanning electron microscopy, X-ray photoelectron spectroscopy, and an image analyzing microscope. The surface roughness was evaluated by atomic force microscopy and a profilometer. The contact angle and surface energy were analyzed. Cell adhesion, cell proliferation, and alkaline phosphatase activity were evaluated using mouse MC3T3-E1 cells.

The anodized Ti group had a more porous and thicker layer on its surface. The surface roughness of the two groups measured by the profilometer showed no significant difference ( P >0.001). The anodized Ti dioxide (TiO₂) surface exhibited better corrosion resistance and showed a significantly lower contact angle than the machined Ti surface ( P >0.001). Although there was no significant difference in the cell viability between the two groups ( P >0.001), the anodized TiO₂ surface showed significantly enhanced alkaline phosphatase activity ( P <0.001).

These results suggest that the surface modification of Ti by anodic oxidation improved the osteogenic response of the osteoblast cells.