Several methods have been used for regeneration of tissue lost by periodontal disease. Subepithelial connective tissue graft technique, one of the technniques of mucogingival surgery, is used for the regeneration in esthetic problems such as recession, and denuded root coverage.

This study is performed to evaluate the healing process and the regeneration and reattachment of periodontal tissue, including the reconstruction of junctional epithelium, and connective tissue.

Alveolar defects in five adult dogs were treated with periodontal surgery and were attained by removing the marginal alveolar bone by 4×3mm from CEJ in the labial side of incisors, and root surfaces were planed.

The experimental sites were divided into two groups as follows.

1. root planing alone(control group) 2. with connective tissue graft(Experimental Group)

In the two groups flaps were positioned and sutured tightly, the healing processes were observed and were histologically compared with each other after 2days, 4days, 1week, 2weeks, 4weeks.

The results were obtained as follows :

In the two groups blood clots were observed as early as 2 and 4 days, and were resorbed at 1 week.

In the two groups moderate inflammation was observed as early as 2 and 4 days, decreased at 1 and 2 weeks, and disappeared at 4 weeks.

Junctional Epithelium migration was more significant in the control group, and was restrained by graft materials in the experimental group.

Features of connective tissue fiber attachment partially showed the parallel pattern in the two groups from 2 weeks, and entirely from 4weeks.

Anastomosis, between graft and connective tissue, appeared from 4 days in the experimental group and the border between them was not discriminated at 4weeks.