文章基本信息

标题：Pro-inflammatory cytokine expression in human gingival fibroblasts by Tannerella forsythia whole bacteria, membrane proteins, and lipopolysaccharide
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作者：Kim, Jung-Eun ; Lee, Sung-Hoon ; Choi, Bong-Kyu 等
期刊名称：The Journal of the Korean Academy of Periodontology
印刷版ISSN：0250-3352
出版年度：2008
卷号：38
期号：3
页码：543-550
DOI：10.5051/jkape.2008.38.3.543
语种：Korean
出版社：Korean Academy of Periodontology
摘要：Purpose
The purpose of this study was to investigate induction of cytokine expression in human gingival fibroblasts (HGFs) by whole cell and the components of T. forsythia .
Material and Methods
After HGFs were treated with lipopolysaccharide (LPS), membrane protein isolated from T. forsythia or culture media of T. forsythia , the induction of interleukin (IL)-1, IL-6 and IL-8 was examined with real-time PCR and ELISA. Their induction ability of cytokines was compared with whole bacteria.
Result
The expression of IL-6 and IL-8 was significantly induced in HGFs by whole bacteria and membrane protein. The expression of IL-1beta was induced by membrane protein of T. forsythia , not by whole bacteria. LPS and condition media of T. forsythia slightly activated HGFs.
Conclusion
The membrane protein of T. forsythia could be one of virulence factors.
关键词：Tannerella forsythia; Virulence factors; Human gingival fibroblasts