文章基本信息
- 标题：Effects of H2O2 and chlorhexidine on MMP-1, TIMP-1,2, Type 1 collagen, fibronectin and UNCL expressions in human periodontal ligament fibroblasts
- 本地全文：下载
- 作者：Choi, Seong-Mi ; Jang, Hyun-Seon ; Kim, Byung-Ock 等
- 期刊名称：The Journal of the Korean Academy of Periodontology
- 印刷版ISSN：0250-3352
- 出版年度：2008
- 卷号：38
- 期号：4
- 页码：645-656
- DOI：10.5051/jkape.2008.38.4.645
- 语种：Korean
- 出版社：Korean Academy of Periodontology
- 摘要：Purpose
To evaulate the effects of chlorhexidine and H2O2 on matrix metalloproteinase-1 (MMP-1), tissue inhibitor of metalloproteinase(TIMP-1, TIMP-2), Type 1 collagen, fibronectin and UNCL expressions in human periodontal ligament fibroblasts (hPDLF).
Materials and Methods1.2×10-1%, 1.2×10-2% and 1.2×10-3% CHX and 3×10-3%, 3×10-4% and 3×10-5% H2O2 and mixture of CHX and H2O2 were applied to hPDLF for 1 min and 30 min. The mRNA expressions of MMP-1, TIMP-1 and 2, Type 1 collagen, fibronectin and UNCL in hPDLF were analysed by RT-PCR.
ResultsThe result were as follows:
The expression of UNCL mRNA was higher than that of other mRNAs.
1.2×10-3 % CHX increased mRNA expressions of hPDLF as application time increased.
H2O2 lower than 3×10-3 % increased expression of UNCL mRNA, and did not decrease mRNA expression of hPDLF.
hPDLF treatment with 1.2×10-1 % CHX (with or without H2O2) resulted in no gene expression.
hPDLF treatment with 1.2×10-2 % CHX (with or without H2O2) for 30 minutes resulted in no gene expression.
ConclusionBecause low concentration of CHX and H2O2 increased UNCL mRNA expression of hPDLF, low concentraction of CHX and H2O2 may have an antioxidative effect.
- 关键词：Chlorhexidine; H2O2; human periodontal ligament fibroblasts
