文章基本信息

标题：Effect of Zoledronate on the Expression of Vascular Endothelial Growth Factor-A by Articular Chondrocytes and Synovial Cells: An in Vitro Study
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作者：Yi, Jin Woong ; Lee, Woo-Suk ; Kim, Sang-Bum 等
期刊名称：Journal of Bone Metabolism
印刷版ISSN：2287-6375
出版年度：2014
卷号：21
期号：4
页码：249-255
DOI：10.11005/jbm.2014.21.4.249
语种：English
出版社：The Korean Society for Bone and Mineral Research
摘要：Background
The aim of this in vitro study was to determine the effect of zoledronate, which is frequently used to treat osteoporosis, on osteoarthritis by analyzing zoledronate-induced expression of vascular endothelial growth factor-A (VEGF-A) in chondrocytes and synovial cells.
Methods
After chondrocytes and synovial cells were separated and cultured, zoledronate was added, and VEGF-A and pigment epithelium-derived factor (PEDF) expression were quantified by real-time polymerase chain reaction and Western blotting.
Results
There was no significant difference in the expression of VEGF-A mRNA in chondrocytes between the zoledronate group and the control group on the 8th day of culture. The expression of both VEGF-A and PEDF mRNA in synovial cells was significantly decreased in the zoledronate group ( P <0.05).
Conclusions
Zoledronate decreases the expression of VEGF-A in synovial cells and may affect the development and progression of osteoarthritis.
关键词：Chondrocytes; Osteoarthritis; Synovial cells; Vascular endothelial growth factor-A; zoledronate