期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2015
卷号：112
期号：2
页码：512-517
DOI：10.1073/pnas.1413291112
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：SignificanceEffector CD8+ T-cell differentiation is essential for protective immunity. Investigating specific genes in this process by knockdown with RNAi is challenging because naive T cells are refractory to viral transduction. To overcome this obstacle, we developed a novel strategy to knock down genes in naive CD8+ T cells by creating bone marrow chimera from hematopoietic progenitors transduced with an inducible shRNA. This approach enabled inducible in vivo gene knockdown in any cell type developed from this progenitor pool. We applied this strategy to show that the transcription factor BATF is essential for initial commitment to effector differentiation but becomes dispensable by 72 h. This approach now enables the study of gene function in vivo in cells of hematopoietic origin otherwise refractory to viral transduction. The differentiation of effector CD8+ T cells is critical for the development of protective responses to pathogens and for effective vaccines. In the first few hours after activation, naive CD8+ T cells initiate a transcriptional program that leads to the formation of effector and memory T cells, but the regulation of this process is poorly understood. Investigating the role of specific transcription factors (TFs) in determining CD8+ effector T-cell fate by gene knockdown with RNAi is challenging because naive T cells are refractory to transduction with viral vectors without extensive ex vivo stimulation, which obscures the earliest events in effector differentiation. To overcome this obstacle, we developed a novel strategy to test the function of genes in naive CD8+ T cells in vivo by creating bone marrow chimera from hematopoietic progenitors transduced with an inducible shRNA construct. Following hematopoietic reconstitution, this approach allowed inducible in vivo gene knockdown in any cell type that developed from this transduced progenitor pool. We demonstrated that lentivirus-transduced progenitor cells could reconstitute normal hematopoiesis and develop into naive CD8+ T cells that were indistinguishable from wild-type naive T cells. This experimental system enabled induction of efficient gene knockdown in vivo without subsequent manipulation. We applied this strategy to show that the TF BATF is essential for initial commitment of naive CD8+ T cells to effector development but becomes dispensable by 72h. This approach makes possible the study of gene function in vivo in unperturbed cells of hematopoietic origin that are refractory to viral transduction.
关键词：CD8 T cell ; RNAi ; transcription factor ; BATF
