期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2015
卷号：112
期号：3
页码：E297-E302
DOI：10.1073/pnas.1424028112
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：SignificanceSuper enhancers (SEs) are enhancer-dense regions found near genes that play key roles in determining cellular identity. Using global nuclear run-on sequencing (GRO-Seq), we find extensive regulation of enhancer RNAs (eRNAs) within SEs in response to lipopolysaccharide (LPS) treatment in macrophages. Both activation and repression of gene expression are associated with SEs and eRNA transcription dynamics. Furthermore, we find that each SE acts as a single regulatory unit within which eRNA and genic transcripts are coordinately regulated. We also find that transcription factor (TF) composition within an SE determines regulatory properties of each SE and associated eRNAs. We propose that signal-dependent SEs and their eRNAs function as molecular rheostats integrating the binding profiles of key regulators to produce dynamic profiles of gene expression. Enhancers are critical genomic elements that define cellular and functional identity through the spatial and temporal regulation of gene expression. Recent studies suggest that key genes regulating cell type-specific functions reside in enhancer-dense genomic regions (i.e., super enhancers, stretch enhancers). Here we report that enhancer RNAs (eRNAs) identified by global nuclear run-on sequencing are extensively transcribed within super enhancers and are dynamically regulated in response to cellular signaling. Using Toll-like receptor 4 (TLR4) signaling in macrophages as a model system, we find that transcription of super enhancer-associated eRNAs is dynamically induced at most of the key genes driving innate immunity and inflammation. Unexpectedly, genes repressed by TLR4 signaling are also associated with super enhancer domains and accompanied by massive repression of eRNA transcription. Furthermore, we find each super enhancer acts as a single regulatory unit within which eRNA and genic transcripts are coordinately regulated. The key regulatory activity of these domains is further supported by the finding that super enhancer-associated transcription factor binding is twice as likely to be conserved between human and mouse than typical enhancer sites. Our study suggests that transcriptional activities at super enhancers are critical components to understand the dynamic gene regulatory network.
