A positive attitude towards physical education (PE) is an important component of students’ engagement in classes and extracurricular physical activities. Relying on students’ interests when planning the class work can contribute to the formation of such an attitude. The research was aimed at verifying the effects of the experimental programme “Inclusion of music in Physical Education classes” on the formation of students’ attitude towards PE. An experiment with parallel groups that lasted for 26 classes was applied in the research on the sample of 141 primary school students attending the seventh grade. In the initial and final testing two instruments for measuring the attitudes were applied: the Students’ Attitudes toward Physical Education - SATPE and the Connotative Differential (CD-15). In the final testing, the experimental group also completed the questionnaire on students’ assessment of classes with and without music. The analysis of variance has shown that the experimental group achieved considerably higher scores at the final testing compared to the initial on the cognitive and conative subscales of the CD-15 instrument. This change was not linked to gender, PE grade and involvement in music. It has been shown that students in the experimental group assessed more positively the classes accompanied by music compared to the classes without music, measured by the questionnaire on students’ assessment of classes with and without music. It can be concluded that the application of music has influenced the development of a more positive attitude towards PE, observed via the cognitive and conative dimension, which points to the fact that it is justifiable and desirable to use music in PE education. [Projekat Ministarstva nauke Republike Srbije, br. 179018 i br. 47015]