PURPOSE: to measure the noise levels in different shifts of Hospital São Paulo's (HSP) laundry and provide information on health and hearing conservation to the employees and supervisors of the sector. METHODS: this is a descriptive transversal study. The noise levels were checked using a Sound Pressure Meter in the "Clean Area" of the laundry, in three periods of the day: morning (between 7am and 8am), afternoon (between 12pm and 1pm) and night (between 5pm and 6pm), in three different days and each measurement lasted five minutes. The examiners also checked the use of Personal Protection Equipment (PPE) by the employees. RESULTS: we observed 11 machines (skylarks, dryers and washers) in the sector, which contribute to an important variation of noise levels in the laundry (70 to 101 dB) in the checked periods. The use of PPE by employees was rare, observing the use by only six employees (12.76%). CONCLUSIONS: regardless of the shift, Hospital São Paulo laundry's employees, who do not effectively use PPEs, are daily exposed to high levels of sound pressure and as the number of machines in operation increased, the noise levels were also higher.