PURPOSE: to evaluate the hearing threshold in the conventional frequencies and high frequencies, and the integrity of the acoustic reflex, in subjects exposed to fuels. METHOD: attendants of three gas stations were evaluated in Santa Maria/RS. After the adaptation to the inclusion criteria, the sample was composed of 24 subjects. It was used the Pure Tone Audiometry, high frequency audiometry and acoustic immitance assessment to evaluate the sample. The time of exposition ranged from one to seventeen years. The control group was composed by 24 not exposed subjects. RESULTS: the average of the thresholds of the PTA and of the high frequency audiometry was superior in the study group in all the tested frequencies. A statistically significant difference of thresholds was verified on the frequencies of 0,5 (p=0,004), 2 (p=0,001), 3 kHz (p=0,025), and frequencies of 9 (p=0,007) e 10 kHz (p=0,026). In 16, 18, 20 kHz a greater absence of responses was observed in the study group (p>0, 05). Also it was observed a greater absence of acoustic reflexes (ipsilateral and contralateral) at the study group, on the right ear. On the left ear, there was no difference between the groups, for the occurrence of ipsilateral reflex. The absence of the contralateral reflex was superior in the study group on all frequencies tested. CONCLUSION: individuals exposed to fuels with normal hearing thresholds may have cochlear and central alterations.