Stress towards diabetes and its treatment is determinant in treatment adherence, metabolic control and quality of life in adolescents. This study aimed to validate the Questionnaire of Stress on Diabetes- Revised (QSD-R), in a sample of 256 adolescents with type 1 diabetes. The results showed psychometric qualities that allow the use of QSD-R with Portuguese adolescents with diabetes. The questionnaire reveals an internal consistency similar to the original version (alpha of .93), consisting of 40 items organized into six components: Somatic complaints (alpha of .81), Stress and in school and social activities (alpha of .82), Anxiety / Depression (alpha of .77), Stress in the face of hypoglycemia (alpha of .79), Doctor-patient relationship (alpha of .75), Treatment regimen (alpha of .72). In the Portuguese version of the QSD-R, the marital relationship and the work stress scales were eliminated and items related to stress in school activities were added. The external validity revealed QSD-R to be correlated with treatment adherence (r = .17, p = .03) and quality of life (r = .55, p = .0001). No differences between teenagers of both sexes were found as well as variation in stress levels, according to age.