标题：Standardization of Protocol for Pre-treatment, Surface Sterilization, Regeneration, Elongation and Acclimatization of Chrysanthemum morifolium Ramat
摘要：In the present study we have developed proficient in vitro regeneration protocol from the ray florets of chrysanthemum cv. Thai Chen Queen to isolate the novel mutants. The maximum survival percentage (89.44) of cultures was found when the ray florets were per-treated with mancozeb-45 (0.1%)+carbendazim (0.1%)+8-HQC (200 mg/L) for 3 hours. Culture establishment was found best (91.04%) when the ray florets surface sterilized with HgCl2 (0.1%) for duration of four minutes. Murashige and Skoog (MS) media supplemented with 4 mg/L BAP and 0.1 mg/L NAA was found to the best for regeneration percentage (93.33), number of microshoots per explants (5.67) and minimum days required for shoot regeneration (30.67). The regenerated plantlets were transferred on the MS media supplemented with GA3 0.50 mg/L was found to the best for shoot elongation after 30 days of elongation. The successful acclimatization of elongated plantlets was achieved by transferring them into glass jar with polypropylene cap filled with peat+soilrite (1:1). The hardening of plantlets was completed after 25 days of acclimatization and then plants were transferred to greenhouse for flowering.
