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  • 标题:Sarin Assay using Acetylcholinesterases and Electrochemical Sensor Strip
  • 本地全文:下载
  • 作者:Miroslav Pohanka ; Jiri Binder ; Kamil Kuca
  • 期刊名称:Defence Science Journal
  • 印刷版ISSN:0976-464X
  • 出版年度:2009
  • 卷号:59
  • 期号:3
  • 页码:300-304
  • DOI:10.14429/dsj.59.1525
  • 语种:English
  • 出版社:Defence Scientific Information & Documentation Centre
  • 摘要:An electrochemical sensor strip was used for sarin assay. Three different acetylcholinesterases (AChEs) were chosen as promising recognition elements. viz., human recombinant, electric eel, and bovine erythrocytes origin. Human recombinant AChE seems to be the most sensitive to inhibition by sarin when the achieved limit of detection (0.45×10-8 mol/l) and IC50 [(9.77± 8.08)×10-6 mol/l] are considered. On the contrary, AChE from bovine erythrocytes proved to reach highest IC50 (5.37± 4.52)×10-7 mol/l and the one from electric eel reached the highest limit of detection 0.93×10-8 mol/l. From the AChEs tested as biorecognition element, human recombinant seems to be the best for construction of new ChE detectors. Defence Science Journal, 2009, 59(3), pp.300-304 , DOI:http://dx.doi.org/10.14429/dsj.59.1525
  • 关键词:sarin;biosensor;detection;organophosphate;AChE;amperometric;nerve agent
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